This will reveal common principles and special adaptations. In parallel we will study selective autophagosome formation in human cells.

We will use cargo mimetics or prApe1 complexes in combination with purified autophagy proteins and vesicles to reconstitute the process and so determine which factors are both necessary and sufficient for autophagosome formation, as well as elucidating their mechanism of action. cerevisiae that mediates the constitutive delivery of the prApe1 enzyme into the vacuole. To this end we will focus on the cytoplasm-to-vacuole-targeting pathway in S.

In particular, we will focus on selective autophagy and determine how the autophagic machinery generates an autophagosome that selectively contains the cargo. We will use a combination of biochemical and cell biological approaches to elucidate the choreography and mechanism of these core factors. Many core factors required for autophagosome formation have been identified but the order in which they act and their mode of action is still unclear. Autophagy thereby protects the organism from pathological conditions such as neurodegeneration, cancer and infections. These are formed during autophagy and mediate the degradation of cytoplasmic substances within the lysosomal compartment. Summary I propose to study how eukaryotic cells generate autophagosomes, organelles bounded by a double membrane. United Kingdom (458) Apply United Kingdom filter.

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